研究内容

基礎研究

１．下顎歯肉癌原発巣と頸部リンパ節転移巣から樹立した細胞株における生物学的特性と転移関連遺伝子の解析

これまで転移に関する研究報告は、切除物の原発巣と転移巣を組織学的に検討したものがほとんどであった。本研究では、同一患者の原発巣と転移巣から細胞株を樹立することに成功し、その2つの癌細胞株について、網羅的な遺伝子解析を行い、転移関連遺伝子について生物学的特性を踏まえて検討した。
　原発巣（WK2）と比較して、転移巣（WK3F）では増殖能・遊走能・浸潤能が高かった。また、in vivo においても腫瘍形成能、転移能ともに高く、WK3F は転移巣より樹立した細胞株としての性質を十分保持していることが確認された（右の図表）。
　マイクロアレイにより転移関連遺伝子候補として MAGEC1、MMP7、SNAI1、MACC1、HTRA1が挙げられた。これらは転移巣の癌細胞の特性（転移機構）を解明する上で重要であり、治療の標的因子となりうることが示唆された。

臨床研究

1．顎位の変化が全身に与える影響

これまで、顎位の変化は呼吸、発音、姿勢など様々なことに影響を与えると考えられていましたが、明らかな関係はわかりませんでした。当科では、様々な方法を用いて、その関係を明らかにしています。

研究業績(論文)

2017年

1. Preoperative chronic sinusitis as significant cause of postoperative infection and implant loss after sinus augmentation from a lateral approach. Kozuma A, Sasaki M, Seki K, Toyoshima T, Nakano H, Mori Y. Oral Maxillofac Surg, 2017.

2. Pediatric Treatment-Resistant Nonbacterial Osteomyelitis of the Mandible Associated with SAPHO Syndrome. Kamata YU, Yamada T, Sumida T, Nakano H, Sugiyama G, Nakashima A, Mori Y. Journal of Dental and Oral Health, 2017

3. The relationship between lateral displacement of the mandible and scoliosis. Nakashima A, Nakano H, Yamada T, Inoue K, Sugiyama G, Kumamaru W, Nakajima Y, Sumida T, Yokoyama T, Mishima K, Mori Y Oral Maxillofac Surg, 2017

4. Histologic Evaluation of Bone Regeneration using Titanium Mesh Prepared by Selective Laser Meiting Technique. Yamamoto K, Yamaguchi S, Matsushita T, Mori S, Kitagaki H, Yoshimura H, Sano K, Susano A, Nakajima Y, Nakano H, Ueno T. J.of Hard Tissue Biology, 2017.

5. Intermaxillary Fixation of Mandibular Fractures using a Bilayer Thermoforming Plate. Hayase T, Nakajima Y, Kato-Kogoe N, Nakano H, Sano K, Yoshimura H, Ariyoshi Y, Ueno T. J.of Hard Tissue Biology, 2017

6. Quantitative Analysis of Velopharyngeal Movement by Applying Principal Component Analysis to Range Images Produced by a Three-Dimensional Endoscope. Nakano A, Mishima K, Shiraishi M, Umeda H, Nakano H ,Ueyama Y. Dentistry Journal, 2017

7. Deregulation of Nicotinamide N-Methyltransferase and Gap Junction Protein Alpha-1 Causes Metastasis in Adenoid Cystic Carcinoma. Ishibashi K, Ishii K, Sugiyama G, Sumida T, Sugiura T, Kanata Y, Seki K, Fujinaga T, Kumamaru W, Kobayashi Y, Hiyake N, Nakano H, Yamada T, Mori Y. Anticancer Res, 2017. in print.

8. Regulation of β-catenin phosphorylation by PR55β in adenoid cystic carcinoma.Ishibashi, K. Ishii, K. Sugiyama, G. Kamata, Y. Suzuki, A. Ohyama, Y. Kumamaru, W. Kiyoshima, T. Nakano, H. Sumida, T. Yamada, T. Mori. Cancer genomics proteomics. 2017. in print.

2016年

1. High Le Fort1 osteotomy for correction of mid-face deformity in Crouzon syndrome. Nakajima Y, Nakano H, Sumida T, et al. Congenital Anomalies. 2016

2. The Contribution of Dental Implants to Functional Artificial Restoration after Treatment of Oral Cancer. Kobayashi Y, Suimida T, Ishikawa A, et al. Anticancer Research 36.2016.

3. Estrogen Enhances Malignant Phenotypes in Human Salivary Adenoid Cystic Carcinoma Cells. Sumida T, Ishikawa A, Kamata YU, et al. Anticancer Research 36, 2016.

4. 顎口腔領域への転移性腫瘍についての臨床的検討. 矢内雄太, 大山順子, 窪田泰孝, 他. 日本口腔外科学会雑誌. 62(4): 199-204. 2016

5.　Objective validity of an implant-retained overdenture with a ball attachment system after marginal mandibulectomy. Murase R, Ishikawa A, Sumida T, et al. Br J Oral Maxillofac Surg. doi: 10.1016/j.bjoms.2015.11.017. 2015.

6. Primary wound closure after tooth extraction for prevention of medication-related osteonecrosis of the jaw in patients under denosumab. Matsumoto A, Sasaki M, Schmelzeisen R, Oyama Y, Mori Y, Voss PJ. DOI: 10.1007/s00784-016-1762-y. 2016.

7. Introduction of ID2 Enghances INvasiveness in ID2-null Oral Squamous Cell Carcinoma Cell via the SNAIL Axis. Kamata YU, Sumida T, Kobayashi Y, Ishikawa A, Kumamaru W, Mori Y. Cancer Genomics Proteomics13(6):493-497,2016.

8.Blockade of Androgen-induced Malignant Phenotypes by Flutamide Administration in Human Salivary Duct Carcinoma Cells. Anticancer Res. 36(11); 6071-6075, 2016.

9.ICD1 ControlsAggresiveness of Salivary Gland Cancer Cells via Crosstalk of IGF and AKT Pathways. Anticancer Res. 36(8); 3865-3870, 2016.

10.Involvement of the T-box transcription factor Brachury in early-stage embryonic mouse salivary gland. Hayashi K, Ikari T, Sugiyama G, Ohyama Y, KUmamaru W, Shirasuna K, Mori Y. Biochem Biophys Res Commun 477(4); 819-819, 2016.

11.Simulation of the Estrogen Axis Induces Epithelial-Mesenchymal Transition in Human Salivary Cancer cells. Sumida T, Ishikawa A, Mori Y. Cancer Genomics 13(4); 305-310, 2016.

12. Targeting ID2 ex@ression triggers a more differentiated phenotype and reduces aggressiveness in human salivary gland cancer cells. Sumida T, Ishikawa A, Nakano H, Yamada T, Mori Ym Desprez PY. Genes Cells 21(8); 915-920, 2016.

13. Efficient regulation of branching morphogenesis via fibroblast growth factor receptor 2c in early-stage embryonic mouse salivary glands. Shibuya M, Ikari, Sugiyama G, Ohyama Y, Kumamaru W, Nagano K, SUgiura T, Shirasuna K, Mori Y. Differentitation 2016.

2015年

１.　Hyperbaric Oxygen Stimulates the Proliferation and Differentiation of Raw 264.7 Cells. Sugi T, Sasaki M, Yamashiro T, et al. Open Journal of Stomatoogy. 5(12). DOI: 10.4236-ojst.2015.512037. 2015.

2.　顎下部に発生した乳腺相似分泌癌の１例．石井広太郎，他．日本口腔外科学会雑誌．61(11)：564-569．2015.

3.　Relationships between Airway Morphology and Sleep Breathing Indices in Jaw Deformity Patients. Nakano H, Mishima K, Nakano A, et al. Journal of Sleep Disorders & Therapy. 4(1). DOI: 10.4172/2167-0277.1000187. 2015.

4.　Plasmablastic lymphoma of the upper gingiva in an HIV-negative elderly patient. Yamada T, Kitamura N, Sasabe E, et al. Oral and Maxillofacial SUrgery Cases, 1: 19-24. 2015.

5.　A novel measurement method for the morphology of the mandibular ramus using homologous modeling. Inoue K, Nakano H, Sumida T, et al. Dentomaxillofacial Radiology 44:doi: 10.1259/dmfr.20150062. 2015.

6.　Dental implants in oral rehabilitation of a maxillary cancer reconstruction: A case report. Sumida T, Nakano H, Hamakawa H, et al. J Oral Implantol, 　41(6): 737-9. 2015.

7.　Custom-made Titanium Devices as Membranes for Bone Augmentation in Implant Treatment: Clinical Application and the Comparison with Conventional Titanium Mesh. Sumida T, Otawa N, Kamata YU, et al. J Craniomaxillofac Surg. 43: 2183-2188, 2015.

8.　Double sentinel lymph node mapping with indocyanine green and 99m-technetium-tin colloid in oral squamous cell. Murase R, Tanaka H, Sumida T, et al. International Journal of Oral and Maxillofacial Surgery. 44: 1212-1217, 2015.

9.　Custom-made titanium devices as membranes for bone augmentation in implant treatment: Modeling accuracy of titanium products constructed with selective laser melting. Otawa N, Sumida T, Kitagaki H, et al. Journal of Cranio-Maxillofacial Surgery. 43: 1289-1295, 2015.

10.　A Clinical Investigation of Oral Sarcomas at Multi-institutions over the Past 30 Years. Sumida T, Otawa N, Kamata YU, et al. Anticancer Research. 35: 4551-4555. 2015.

11.　A Novel Function of CD82/KAI1 in Sialyl Lewis Antigen-Mediated Adhesion of Cancer Cells: Evidence for an Anti-Metastasis Effect by Down-Regulation of Sialyl Lewis Antigens. Yoshihama N, Yamaguchi K, Chigita S, et al. PLoS One. 10(4); doi: 10.1371/journal.pone.0124743. 2015.

12.　miR-203 Inhibits Frizzled-2 Expression via CD82/KAI1 Expression in Human Lung Carcinoma Cells. Mine M, Yamaguchi K, Sugiura T, et al. PLoS One. 10(7); doi: 10.1371/journal.pone.0131350. 2015.

13.　Tomosyn is a novel Akt substrate mediating insulin-dependent GLUT4 exocytosis. Nagano K, Takeuchi H, Gao J, et al. Int J Biochem Cell Biol. 62: 62-71. 2015.

14.　Signaling pathway for adiponectin expression in adipocytes by osteocalcin.OtaniT,Mizokami A, Hayashi Y, et al. Cell Signal. 27(3): 532-44. 2015.

現在行っている臨床研究

1．顎変形症における咀嚼筋の分子病理学的検討

2. 腺様嚢胞癌におけるPR55βの発現量と悪性度の関連性に関する研究